deletions | additions       diff --git a/Results_and_Discussion.tex b/Results_and_Discussion.tex  index 4bb31fc..fac784e 100644  --- a/Results_and_Discussion.tex  +++ b/Results_and_Discussion.tex 
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\subsection{$^{13}$C from cellulose assimilated by canonical cellulose-degrading and uncharacterized microbial lineages in many phyla including \textit{Chloroflexi} and \textit{Verrucomicrobia}}  Only 2 and 5 OTUs were found to have incorporated $^{13}$C from labeled cellulose at days 3 and 7, respectively. At days 14 and 30, however, 42 and 39 OTUs were found to incorporate $^{13}$C from cellulose into biomass. An average 16\% of the $^{13}$C-cellulose added was respired within the first 7 days, 38\% by day 14, and 60\% by day 30. A \textit{Cellvibrio} and \textit{Sandaracinaceae} OTU assimilated $^{13}$C from cellulose at day 3. Day 7 responders included the same \textit{Cellvirio} responder at day 3, a \texit{Verrucomicrobia} OTU and three \textit{Chloroflexi} OTUs. 50\% of Day 14 responders belong to Proteobacteria (66\% Alpha-, 19\% Gamma-, and 14\% Beta-) followed by 17\% \textit{Planctomycetes}, 14\% \textit{Verrucomicrobia}, 10\% \textit{Chloroflexi}, 7\% \textit{Actinobacteria} and 2\% \textit{Cyanobacteria}. \textit{Bacteroidetes} OTUs begin to incoporate $^{13}$C from cellulose at dat 30 (13\% of day 30 responders). Other day 30 responding phyla include \textit{Proteobacteria} (30\% of day 30 responders; 42\% Alpha-, 42\% Delta, 8\% Gamma-, and 8\% Beta-), \textit{Planctomycetes} (20\%), \textit{Verrucomicrobia} (20\%), \textit{Chloroflexi} (13\%) and \textit{Cyanobacteria} (3\%). \textit{Proteobacteria}, \textit{Verrucomicrobia}, and \textit{Chloroflexi} had relatively high numbers of responders with heavy response across multiple time points (ref l2fc figure).  Proteobacteria \textit{Proteobacteria}  represent 47\% of all cellulose responders responding OTUs  identified. Of those, Cellvibrio \textit{Cellvibrio}  accounted for 5\% of all Proteobacterial responders responding OTUs  detected (\href{https://www.authorea.com/users/3537/articles/3612/master/file/figures/l2fc_fig1/l2fc_fig.pdf}{Figs. 2}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/l2fc_fig_pVal/l2fc_fig_pVal.png}{S4}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/cellulose_resp_profiles/cellulose_resp_profiles.png}{S6}). Cellvibrio \textit{Cellvibrio}  was one of the first identified cellulose degrading bacteria and was originally described by Winogradsky in 1929 who named it for its cellulose degrading abilities \cite{boone2001bergeys}. Other prominent cellulose degrading Proteobacteria, Stenotrophomonas and Devosia have previously been demonstrated in degrading cellulose \cite{Trujillo_Cabrera_2012, Verastegui_2014}. Brevundimonas has not previously been identified as a cellulose degrader, but has been show to degrade cellouronic acid, an oxidized form of cellulose \cite{Tavernier_2008}. Verrucomicrobia, a phylum found to be ubiquitous and in high abundance in soil \cite{Fierer_2013}, have been noted for degradation of polysaccharides in soil, aquatic, and anoxic rice patty soils \cite{Fierer_2013,Herlemann_2013,10543821}. In this study, Verrucomicrobia comprise ~11\% of the total cellulose responder OTUs detected (\href{https://www.authorea.com/users/3537/articles/3612/master/file/figures/l2fc_fig1/l2fc_fig.pdf}{Figs. 2}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/l2fc_fig_pVal/l2fc_fig_pVal.png}{S4}, \href{https://authorea.com/users/3537/articles/8459/master/file/figures/cellulose_resp_profiles/cellulose_resp_profiles.png}{S6}) most of which belong to the uncultured FukuN18 clade originally identified in freshwater lakes \cite{Parveen_2013}. Yet the largest enrichment measured (l2fc = 3.7) during the whole time series for \textsuperscript{13}C-cellulose assimilation was by an uncultured Verrucomicrobia in the Verrucomicrobiaceae family on d14 (\href{https://authorea.com/users/3537/articles/3612/master/file/figures/bacteria_tree/bacteria_tree.png}{Fig. 4}).