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\textit{Gluconoacetobacter xylinus} was grown up on Heo and Son 0.1\% glucose agar plates (using ^{12}C-glucose) at 30°C without inositol \cite{Heo_Son_2002}. Heo and Son liquid minimal media was made with 0.1\% glucose (one batch with 12^{C}- and another with 13^{C}-glucose). ^{13}C-glucose).  To increase surface area for cellulose growth, 100mL of the media were added to individual 1L Erlenmeyer flasks. Each aliquot of media was inoculated with three isolated colonies of \textit{Gluconoacetobacter xylinus} from the Heo and Son plate previously grown. Flasks were kept static in the dark at 30°C for 2-3 weeks until thick cellulose pellicule had formed. To wash culture from cellulose, two parts 1\% alconox was added to each flask and autoclaved. Cellulose pellicules were rinsed with deionized (DI) water (1L) until no suds were produced (~10 rinses). Each pellicule was put in 1L of DI water for overnight dialysis. Pellicules were rinsed (3 washes) with DI water twice a day for 2-3 days to ensure no detergent or culture media remained associated with cellulose. Harvested pellicules were dried overnight (60°C) and then cut into pieces and ground using ball grinder until desired size range (53um - 250um) was achieved (checked by sieve). Size range was based on particulate organic matter to emulate how microbes may experience cellulose in the environment and for even distribution in microcosms.