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\textit{Gluconoacetobacter xylinus} was grown up on Heo and Son 0.1\% glucose agar plates (using 12^{C}-glucose) at 30°C without inositol (Heo and Son 2002). Heo and Son liquid minimal media was made with 0.1\% glucose (one batch with 12C- 12^{C}-  and another with 13C-glucose). 13^{C}-glucose).  To increase surface area for cellulose growth, 100mL of the media was sterilely added to individual 1L Erlenmeyer flasks. Each aliquot of media was inoculated with three isolated colonies of Gluconoacetobacter xylinus from Heo and Son plate. Flasks were kept static in the dark at 30°C for 2-3 weeks until thick cellulose pellicule had formed.