Chuck results edits  over 9 years ago

Commit id: 2042491213a6dd6844855f8fa6c9326fb57efd19

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including Chloroflexi and Verrucomicrobia}   Isotope incorporation by an OTU is revealed by enrichment of the OTU in heavy  CsCl gradient fractions containing $^{13}$C labeled DNA relative to heavy  fractions from control gradients (no containing no  $^{13}$C labeled DNA). DNA. We refer  to OTUs that putatively incorporated $^{13}$C into DNA from an isotopically  labeled substrate as a substrate ``responder''.  Only 2 and 5 OTUs were found to have incorporated $^{13}$C from labeled cellulose $^{13}$C-cellulose  at days 3 and 7, respectively. At days 14 and 30, however, 42 and 39 OTUs were found to incorporate $^{13}$C from cellulose $^{13}$C-cellulose  into biomass. An average 16\% of the $^{13}$C-cellulose added was respired within the first 7 days, 38\% by day 14, and 60\% by day 30. A \textit{Cellvibrio} and \textit{Sandaracinaceae} OTU assimilated $^{13}$C from cellulose $^{13}$C-cellulose  at day 3. Day 7 $^{13}$C-cellulose responders included the same \textit{Cellvibrio} responder as day 3, a \textit{Verrucomicrobia} OTU and three \textit{Chloroflexi} OTUs. 50\% of Day 14 responders belong to \textit{Proteobacteria} (66\% Alpha-, 19\% Gamma-, and 14\% Beta-) followed by 17\% \textit{Planctomycetes}, 14\% \textit{Verrucomicrobia}, 10\% \textit{Chloroflexi}, 7\% \textit{Actinobacteria} and 2\% cyanobacteria. \textit{Bacteroidetes} OTUs begin to incoporate $^{13}$C from cellulose at day 30 (13\% of day 30 responders). Other day 30 responding phyla include \textit{Proteobacteria} (30\% of day 30 responders; 42\% Alpha-, 42\% Delta, 8\% Gamma-, and 8\% Beta-), \textit{Planctomycetes} (20\%), \textit{Verrucomicrobia} (20\%), \textit{Chloroflexi} (13\%) and cyanobacteria (3\%). \textit{Proteobacteria}, \textit{Verrucomicrobia}, and \textit{Chloroflexi} had relatively high numbers  of responders with heavy response across multiple time points  (Figure~\ref{fig:l2fc}).