Ashley Campbell edited Results & Discussion.tex  over 9 years ago

Commit id: 123d86bc7ad341272054c7206bad02f11cf5f199

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Our study is consistent with carbon degradative succession that has previously been demonstrated \cite{Bastian_2009} (more refs). We demonstrate a rapid decrease in the labile carbon, xylose, confirmed by its \textsuperscript{13}C label incorporation into the microbial community DNA during the first 7 days of the experiment, after which, the label is not detectable in the DNA. Subsequently our data demonstrates a slow degradation of the more recalcitrant, polymeric carbon demonstrated by \textsuperscript{13}C-cellulose label incorporation into the microbial community DNA at 14 and 30 days. We did not observe the \textsuperscript{13}C-cellulose signal leave the DNA within the time limits, 30 days, of our experiment. This degradative succession is also confirmed by isotopic analysis of the soil from the microcosms (Table S1).   We did not observe consistent C utilization at the phylum level although both xylose and cellulose utilization were observed across 7 phyla each revealing a high diversity of bacteria able to utilize these substrates. The high taxonomic diversity may enable substrate metabolism under a broad range of environmental conditions \cite{Goldfarb_2011}. Other studies of microbial communities have observed a positive correlation with taxonomic or phylogenetic diversity and functional diversity \cite{Fierer_2012,Fierer_2013,Philippot_2010,Tringe_2005,Gilbert_2010,Bryant_2012}. The data presented here supports that specific functional attributes can be shared among diverse, yet distinct, taxa while closely related taxa may have very different physiologies \cite{Fierer_2012,Philippot_2010}. This information adds to the growing collection of data suggesting that community membership is important to biogeochemical processes. Furthermore, demonstrates it highlights  a need to examine substrate utilization by discrete microbial taxa within a whole community context to better understand how specific community members function within the whole. The sensitivity of SIP-NGS provides a means to elucidate substrate utilization by discrete microbial taxa with the hope that we can begin to construct a belowground C food web.