Numerous methods exist for analysing sparks in confocal linescans. These employ various different approaches: noise tresholding \cite{R_os_2001,Picht_2007}, wavelet transform \cite{Szab__2010}, etc.
Recently, a method was developed by Tian et al., \cite{Tian_2012}, where fluorescence time trace in each pixel is fitted and provides a practically noise free approximation of the original fluorescence data. This pixel-by-pixel method had, however, several limitations which made it impractical to be used for Ca2+ release event detection.
Here we extend the method by Tian et al., in several ways. The new method allows for Ca2+ release event classification based on pixel-by-pixel denoising of the original signal.