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# Methods  ## Cell isolations isolation  ## Experimental Solutions  ## Confocal Ca2+ imaging  Data acquisition was performed on two confocal setups. Linescan images were obtained on a an  Olympus FluoView 1000 confocal microscope. Framescan images were recorded with a VTInfinity multi-beam confocal microscope recording 512x64 pixel images at 150Hz freqency. ## Computational  The detection algorithm is presented in the Results section.