Ardo Illaste edited figures/fit_linescan/caption.md  about 10 years ago

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Fitting a linescan image. Each time trace in the raw fluorescence signal (a) (A)  is fitted and a denoised image is obtained (b).The (B).The  denoised image is a sum of fitted events (c) (C)  and baseline (d). (D).  ΔF/F0 image (E)  is obtained by dividing the event image (c) (C)  with the baseline (d). (D).  Because the fitted baseline is a function of time, the ΔF/F0 \(\Delta F/F_0\)  image is automatically corrected for temporal changes in background flourescence (e.g., bleaching). (g) Example of a fit from the pixel time course indicated by the black marker on panel (b). (h) (F)  The residual signal obtained when subtracting the fitted image from raw data. Residual values are normally distributed (i). (G).