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The method works by fitting fluorescence signal from each pixel with a transient function. Prior to fitting, candidate regions containing possible events must be detected. This is achieved by modifying a continous wavelet transform based peak detection algorithm by Du et al.,\cite{Du_2006}. We have altered the method to also yield the width of the peak in addition to the location. Specific details of the region detection algorithm are given in the Supplementary Material.  ### Signal fitting  The function used for fitting Ca2+ release events is shown on Figure \ref{fig:f2} \ref{fig:f2}. The shape of the function is described by 4 parameters: amplitude (\(A\)), rise and decay time constants \(\tau_{rise},\tau_{decay}\) and plateau duration \(d\).  ### Clustering  ### Event characterisation