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## Biological results  ### Spark location  ### Waves in the cytosol and SR  When analyzing Ca waves in cardiomyocytes it is customary to deskew them (REF) before averaging the signal in the spatial dimension. This relies on detecting the half maximal fluoresence along each pixel and then straigthening the linescan. This approach can be problematic in case of noisy images or when dealing with several waves in one image.