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### Signal fitting  The function used for fitting Ca2+ release events is shown on Figure \ref{fig:f2}. The shape of the function is described by 4 parameters: amplitude (\(A\)), rise and decay time constants (\(\tau_{rise},\tau_{decay}\)( and plateau duration (\(d\)). An additional parameter (\(t_{max}\)) determines the time when the maximum is reached.   Because it is not know which part of the signal is the event and which is the baseline the first fit also has to estimate the baseline properties.  Signal in the candidate region is fitted with an extended fit function (Figure \ref{fig:fig2}) that also depends on relaxation baseline \(B\) and baseline offset \(C\). The \(C\) paremeter parameter  takes into account the possibility of an elevated background before the release event. ### Clustering  ### Event characterisation