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\label{fit:cluster}
**Classifying detected events.** **A**
Based on the 4 shape parameters (see Figure \ref{fig:fit}), a density Density based clustering algorithm is used to classify pixel events into
categories. Plots show projections of 4-dimensional parameter space. categories based on their shape. Colours represent event categories. Black signifies events which could not be categorized.
**B** Categories obtained from clustering by shape plotted according to event location.
**B** **C** In the second step of classification each shape category is clustered further based on location. Groups of pixel events are obtained that make up a Ca2+ release even (in this case sparks and a wave).
**C** Using only pixel events that were successfully classified **D** All detected sparks visualized according to their center(location of coloured circle) and amplitude (colour of the circle). Events marked with a
new ΔF/F0 image can be built using only black x were not symmetric and not classified
events (compare as sparks. Circle colour shows sparks originating from the same 2um segment. Empty circles are sparks with
\ref{fig:linescan}E) no nearby sparks.