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\label{fig:linescan}  **Fitting a linescan image.** **A** Raw fluorescence signal. **B** Each horizontal line along the temporal axis if fitted with the fitting algorithm. The fitted signal in the denoised image is a sum of fitted events (**C**) and baseline (**D**). ΔF/F0 image (**E**) is obtained by dividing the event image (**C**) with the baseline (**D**). Because the fitted baseline is a function of time, the ΔF/F0 image is automatically corrected for temporal changes in background flourescence (e.g., bleaching). **F** The residual signal obtained when subtracting the fitted image from raw data. **G** Residual values are normally distributed. Histogram of the residual values.