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## Pixel by pixel event classification  The algorithm is presented schematically on figure 1. Each subroutine is explained in detail below.  ### Image preprocessing  The only preprocessing step used is convolving the image with a \((2n+1)\times(2n+1)}\) \((2n+1)\times(2n+1)\)  kernel ### Region detection  The PIPED method works by fitting fluorescence signal from each