Materials and methods

Please provide a detailed account of the methods and materials used for the study in order to allow replication by other researchers. If you have used a standard protocol that is published elsewhere (e.g. Nature Protocols, Current Protocols), a reference may be used. Please include

  • the source of all samples, reagents, antibodies etc.

  • how samples were selectedwhat exclusions were made, if any;

  • what was being measured

  • For processed data, any software used to process the data and, where possible, the source code should be made openly available.

  • For laboratory animal studies, we recommend compliance with the ’Animal Research: Reporting In Vivo Experiments’ (ARRIVE) guidelines, developed by NC3Rs to improve standards of reporting to ensure that the data from animal experiments can be fully scrutinized and utilized.

  • Allowances made, if any, for controlling bias or unwanted sources of variability. Limitations of the datasets.

  • Acronyms and abbreviations must be explained

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