Liisa Hirvonen edited Results.tex  over 8 years ago

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\section{Results}  The measured rotational correlation time increases with solvent viscosity, as expected (Fig~\ref{fig:exampleFits}). A double-exponential fit to the anisotropy yields excellent fit results for all measured proteins; example data sets; the fits results are consistent and largely independent of starting parameters and fitting range, and the residuals are flat without systematic deviations. Example  fits to three Eylea data sets different viscosities for each protein  are shown in Fig~\ref{fig:exampleFits}. The longer rotational correlation times corresponding to the protein rotation were plotted against the viscosity, see Fig~\ref{fig:results}. For each protein the rotational correlation time increases linearly with viscosity, as expected. this yields a straight line.  Gradients of 43.28$\pm$0.12~ns/cP for BSA, 51.47$\pm$0.12~ns/cP for Eylea, 21.40$\pm$0.11~ns/cP for Lucentis and 98.09$\pm$0.04~ns/cP for Avastin were obtained by straight line fits to the data sets using least squares method. Using eq~\ref{eq:R_h}, this yields experimental radius of 3.49$\pm$0.03~nm for BSA, 3.70$\pm$0.03~nm for Eylea, 2.75$\pm$0.04~nm for Lucentis and 4.58$\pm$0.01~nm for Avastin. Summary of the calculated and measured hydrodynamic radii is shown in Table~\ref{table:res}. \begin{table}  \caption{ \label{table:res} Summary of calculated and measured hydrodynamic radii.}  \begin{tabular}{ l c c c c c c }   & BSA & Eylea & Lucentis & Avastin & Eq & Ref \\   & 66.5 kDa & 115 kDa & 48 kDa & 149 kDa & & \\   R$_{\text{min}}$ (nm) & 2.67 & 3.21 & 2.40 & 3.50 & \ref{eq:Erickson} & \cite{Erickson2009} \\  R$_\text{h}^{\text{Wilkins}}$ (nm) & 3.04 & 3.52 & 2.77 & 3.85 & \ref{eq:Wilkins} & \cite{Wilkins1999} \\  R$_\text{h}^{\text{Dill}}$ (nm) & 4.00 & 4.87 & 3.52 & 5.49 & \ref{eq:Dill} & \cite{Dill2011} \\  R$_\text{h}^{\text{meas}}$ (nm) & 3.49 & 3.70 & 2.76 & 4.58 & \ref{eq:R_h} & \\  \end{tabular}  \end{table}