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The \textbf{Purpose:} To measure the  hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab, aflibercept and bevacizumabwere measured  with $\mu$s $\mu$s5  time-resolved fluorescence anisotropy. \textbf{Methods:}  Ruthenium-based dye Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$, whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these drugs in buffer, was used as a label. The hydrodynamic radii were calculated from the rotational correlation times of the Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$-labelled drugs obtained with time-resolved fluorescence anisotropy measurements in buffer/glycerol solutions of varying viscosity. \textbf{Results:}  The measured radii of 2.76$\pm$0.04~nm for ranibizumab, 3.70$\pm$0.03~nm for aflibercept and 4.58$\pm$0.01~nm for bevacizumab agree with calculations based on molecular weight. weight and other experimental measurements.  \textbf{Conclusions:} Time-resolved fluorescence anisotropy is a relatively simple and straightforward method that allows experimental measurement of hydrodynamic radius of individual proteins, and is superior to theoretical calculations which cannot give the required accuracy for a particular protein.  \textbf{Keywords:} Hydrodynamic radius, fluorescence, phosphorescence, time-resolved anisotropy, rotational diffusion