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\end{equation}  where $A_1$ and $A_2$ are the amplitudes and $\phi_1$ and $\phi_2$ the rotational correlation times of the two different components.  The longer A plot of the  rotational correlation timefor each protein obtained from the fitting was plotted  against the viscosity. This viscosity  yields a straight line, from which the hydrodynamic radius $R_h$ of the rotating unit was can be  calculated by combining the Stokes-Einstein-Debye equation (eq~\ref{eq:SED}) with the equation for the volume of a sphere: \begin{equation}  R_h=\sqrt[3]{\frac{3kT}{4\pi}\frac{\phi}{\eta}} \label{eq:R_h}  \end{equation}