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\end{equation}  where $\eta$ is the solvent viscosity, $k$ is the Boltzmann constant and $T$ is the absolute temperature.  Although time-resolved anisotropy measurements are a well established tool in molecular biology, only a few studies report applications in ophthalmology.\cite{McLaughlin2013, Danysh2010} In this study the effective hydrodynamic radii of three important posterior segment drugs -- ranibizumab, aflibercept and bevacizumab -- were measured by $\mu$s time-resolved fluorescene fluorescence  anisotropy and compared to radii calculated from the MW. A well-characterised protein bovine serum albumin (BSA) of comparable size (66.5~kDa) with known radius \cite{Axelsson1978} was also measured to validate the results.