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The hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab, aflibercept and bevacizumab were measured with $\mu$s  time-resolved fluorescence anisotropy.The molecules were labelled with  Ruthenium-based dye, dye Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$,  whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these molecules. drugs in buffer, was used as a label.  The hydrodynamic radii were calculated from the rotational correlation times of the molecules Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$-labelled drugs  obtained with time-resolved fluorescence anisotropy measurements. measurements in buffer/glycerol solutions.  The measured radii of 2.76$\pm$0.04~nm for ranibizumab, 3.70$\pm$0.03~nm for aflibercept and 4.58$\pm$0.01~nm for bevacizumab agree with calculations based on molecular weight.Anisotropy offers an alternative independent means of determining the size of drugs designed for intraocular use and may better reflect ocular drug diffusion than theoretical estimates of size based solely on MW.