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Fluorescence anisotropy measurements were used for measuring the hydrodynamic radii of intravitreal drugs ranibizumab, aflibercept and bevacizumab. The molecules were labelled labeled  with Ruthenium-based dye, whose lifetimein the region  of several hundred ns nanoseconds  is comparable to the rotational correlation time of these molecules. The molecular radii were obtained from the rotational diffusion coefficients correlation times  of the molecules measured with time resolved time-resolved  fluorescence anisotropy measurements. The measured  radii of ranibizumab, aflibercept and bevacizumab were measured as bevacizumab,  2.76$\pm$0.04~nm, 3.70$\pm$0.03~nm and 4.58$\pm$0.01~nm, respectively,which all  agree with calculations based on molecular weight. Anisotropy offers an alternative independent means of determining the size of drugs designed for intraocular use and may better reflect ocular drug diffusion than theoretical estimates of size based solely on MW.