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The hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab, aflibercept and bevacizumab were measured with $\mu$s time-resolved fluorescence anisotropy. Ruthenium-based dye Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$, whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these drugs in buffer, was used as a label. The hydrodynamic radii were calculated from the rotational correlation times of the Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$-labelled drugs obtained with time-resolved fluorescence anisotropy measurements in buffer/glycerol solutions of varying viscosity. The measured radii of 2.76$\pm$0.04~nm for ranibizumab, 3.70$\pm$0.03~nm for aflibercept and 4.58$\pm$0.01~nm for bevacizumab agree with calculations based on molecular weight. \textbf{Keywords:} Hydrodynamic radius, fluorescence, phosphorescence, time-resolved anisotropy, rotational diffusion