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Fluorescence anisotropy measurements were used for measuring the The  hydrodynamic radii of intravitreal anti-VEGF  drugs ranibizumab, aflibercept and bevacizumab. bevacizumab were measured with time-resolved fluorescence anisotropy.  The molecules were labeled with Ruthenium-based dye, whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these molecules. The molecular radii were obtained from the rotational correlation times of the molecules measured with time-resolved fluorescence anisotropy measurements. The measured radii of ranibizumab, aflibercept and bevacizumab, 2.76$\pm$0.04~nm, 3.70$\pm$0.03~nm and 4.58$\pm$0.01~nm, respectively, agree with calculations based on molecular weight. Anisotropy offers an alternative independent means of determining the size of drugs designed for intraocular use and may better reflect ocular drug diffusion than theoretical estimates of size based solely on MW.