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where $\eta$ is the solvent viscosity coefficient, $k$ is the Boltzmann constant and $T$ is the absolute temperature.  Although time-resolved anisotropy measurements are a well established tool in molecular biology, only a few studies report applications in ophthalmology.\cite{McLaughlin2013, Danysh2010, Srikantha2012} In this study the diffusion coefficients and subsequently the effective hydrodynamic radii of three important posterior segment drugs -- aflibercept, bevacizumab ranibizumab, aflibercept  and ranibizumab bevacizumab  -- were measured by Tr-FAIM and compared to radii calculated from the MW. A well-characterised protein BSA of comparable size (66.5~kDa) with known radius \cite{Axelsson1978} was also measured to validate the results.