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\textbf{Purpose:} To measure the hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab, aflibercept and bevacizumab with $\mu$s5 $\mu$s  time-resolved fluorescence anisotropy. \textbf{Methods:} Ruthenium-based dye Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$, whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these drugs in buffer, was used as a label. The hydrodynamic radii were calculated from the rotational correlation times of the Ru(bpy)$_2$(mcbpy-O-Su-ester)(PF$_6$)$_2$-labelled drugs obtained with time-resolved fluorescence anisotropy measurements in buffer/glycerol solutions of varying viscosity.