Yersinia are a diverse genus of gram-negative enterobacteriaceae, three species of which are well defined human pathogens Y.pestis, Y.pseudotuberculosis and Y.enterocolitica. Identifying and gauging the pathogenicity of a given Yersinia species is an important for public health monitoring. Here we have used complementory bioinformatic tools to determine the species of an unknown Yersinia sample using whole genome sequencing, followed by genome annotation to search for known pathogentic genome features in Yersinia. Phylogentic analysis confirmed that the query sequence belonged to the Y.enterocolitica species. The absence of sequence for the pYV plasmid and the ’high-pathogenicity island’, are indicative of a non-pathogenic 1A strain. We also identified genes in the query strain which were not present in other pathogenic Y.enterocolitica 1B biovars. This may be indicative of other functional differences that influence pathogenicity.