Christopher Medway edited Introduction.tex  over 8 years ago

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\section{Introduction}  \textit{Yersinia} is a genus of gram-negative enterobacteriaceae. Of the characterised species of Yersinia, three have been particularly well studied due to their pathogenicity to humans; \textit{Y.pestis}, \textit{Y.pseudotuberculosis} and \textit{Y.enterocolitica}. \textit{Y.pestis} is highly pathogeneic, resulting in a systemic disease ('plague') which affects multiple organ systems; lungs, lymph nodes and blood vessels. Conversly, \textit{Y.pseudotuberculosis} and \textit{Y.enterocolitica} are enteropathogens, primarily affecting the gastrointestinal system where they can cause local inflammation, diarrhea and fever. Furthermore, while \textit{Y.pestis} is transmitted through flea bites, \textit{Y.enterocolitica} and \textit{Y.pseudotuberculosis} infections are primarily the result of consuming contaminated food or water. Other Yersinia species are not thought to be pathogenic to humans.  \textit{Y.entrocolitica} strains are particularly diverse, containing a spectrum of non-pathogenic (1A), mildly-pathogenic (2-5) and pathogenic (1B) biovars., which can be further differentiated based on serotype. Interestinly, while biovar 1A is primarily found in North America, non-pathogenic \textit{Y.enterocolitica} are more common in Japan and Europe \cite{15493818}.  Isolating the genomic features that determine the virulance of Yersinia is of major interest. Perhaps the most well established is the ~70kb pYV plasmid which is common to all pathogenic yersinia. Yersinia, including pathogenic members of \textit{Y.enterocolitica}.  Similarly the yersiniabactin gene cluster, located in the 'high-pathogenicity island', is not evident in non-pathogenic Yersinia \cite{15493818}.\textit{Y.entrocolitica} strains are particularly diverse, containing a spectrum of non-pathogenic (1A), mildly-pathogenic (2-5) and pathogenic (1B) biovars. These biovars can be further characterised by their serotype and on their biochemical profile. Interestinly, while biovar 1A is primarily found in North America, non-pathogenic \textit{Y.enterocolitica} are more common in Japan and Europe \cite{15493818}.  Given the diversity within the Yersinia genus, the ability to quickly identify a Yersinia species from a sample is important for public health. This has been aided by the development of; i) high-throughput sequencing of whole bacterial genomes and, ii) curated databases of genomic features that confer pathogenicity to humans, such as YersinaBase (http://yersinia.um.edu.my/index.php/home/main). In this study we use a range of bioinformatic tools, on whole-genome sequence from an unknown Yersinia sample, in an attempt to correctly identify the species and to gauge the pathogenicity of the Yersinia on human health.