Christopher Medway edited Results and Discussion.tex  over 8 years ago

Commit id: 323e3b25eabbd52f301c3dd33773549778f4e481

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The metabolic flexibility of \textit{Y.enterocolitica} biovars is known to the highly heterogeneous, with biovar 1A displaying the broadest metabolic potential \cite{Reuter}. Several metabolic pathways are known to be specific to certain Yersinia linieges; cobalamine biosynthesis (\textit{cob}), 1,2-propanediol utilisation(\textit{pdu}), tetrathionate respiration (\textit{ttr}) and [NiFe]-hydrogenase complexes (\textit{hyd2/4}).   Comparison of all functional catagories between our query \textit{Y.enterocolitica} biovar and a 'reference pathogenic' Y.enterocolitica strain 8081 (a 1B biovar) isolates a number of exclusive functional domains. Most strikingly, the pathogenic reference is enriched for; i) membrane transport protein, particularly of the type-III sectreation system, and ii) iron-aquasition and metabolism proteins.  Our query strain has a number of metabolic/functional catagories not found in the pathogenic highly-pathogenic  reference; i)Coenzyme B12 biosynthesis ?? (\textit{pduO}), ii)  RTX toxin cluster, iii) ii)  beta-Fimbriae cluster  (type VII secretion system), iv) iii)  Choloylglycine hydrolase and bile hydrolysis, v) iv)  Central meta-cleavage pathway of aromatic compound degradation, vi) v)  D-galactonate catabolism, vii) vi)  Aldehyde dehydrogenase B, viii) vii)  L-fucose utilization**, ix) utilization, viii)  N-Acetyl-Galactosamine and Galactosamine Utilization**, x) Utilization, ix)  Phage packaging machinery, Phage replication and Phage tail proteins and xi)Xanthosine utilization (xap region). \cite{21798805} Interestingly it has been recently found that atypical, pathogenic 1A biovars contain the beta-Fimbriae cluster, Galactosmine utilisation pathways and RTX cluster, suggesting that may may confer some pathogenic potential \cite{21798805}.  Taken together, the phylogenetic analysis and genome annotation suggest that the query sequence is of a non-pathogenic strain (1A) of \textit{Y.enterocolitica}. Whilst not carrying any of the established markers of pathogenicity, the presence of several novel virulance features may confeer upon it a degree of pathogenic potential.