deletions | additions       diff --git a/Materials_and_Methods.tex b/Materials_and_Methods.tex  index cc45e2f..c255a4c 100644  --- a/Materials_and_Methods.tex  +++ b/Materials_and_Methods.tex 
...
have a proportion mean in one class that is statistically different from its  proportion mean in another. This differential abundance could mark an  enrichment of the OTU in either sample class and the direction of the  enrichment is apparent in the sign (positive or negative) of the fold change.Here we use log$_{2}$ of the abundance fold change as our  differential abundance metric.  Differential abundance is was  moderated by  DESeq2 (see \citet{Love_2014})  such that fold change can be used to rank the enrichment of  OTUs by magnitude. that span a wide range of  base abundance.  The DESeq2 RNA-Seq statistical framework has been shown to improve power and specificity when identifying differentially abundant OTUs across sample classes in microbiome experiments \citet{24699258}. The specific DESeq2 \citep{Love_2014} parameters we used were as  follows: All dispersion estimates from DESeq2 were calculated using a local fit