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  **If we combine the total OTUs present in the planktonic communities at each timepoint we note that our study shows X OTUs. This is (Lower - Higher) than the Besemer study and....*** CP-R    While there are only a few studies that attempt to compare biofilm community composition and the overlyng planktonic community abundance those studies that have, have found community composition among the two habitats are unique with very few taxa found in both (Besemer 2007, Besemer 2012, Jackson 2001, Lyautey et al. 2005). This is consistent with our findings in this experimental system with a natural marine planktonic source commmunity. Our study also evaluates algal community composition which showed a similar result suggesting that both the algal and bacterial biofilm communities form from phylogenetically unique organisms that exist in low abundance in surrounding habitat but are readily enriched in the biofilm lifestyle.While our study provides an additional detailed analysis of biofilm community assembly mechanisms consistent with what has been previously reported we also evaluated how resource subsidies potentially alter the seed pool (the plankton) and the biofilm community. Interestingly, biofilm community richness peaked at the intermediate treatment (C:P =100) and appeared to decrease over time although with only two time points it was unclear how pronounced this effect was. Since biomass pools for the plankton and the biofilm increased with increasing carbon subsidies the intermediate peak in OTU richness is consistent with a classic productivity-diversity relationship that has been shown for many ecosystems and communities both microbial and otherwise (REFS). Interestingly, as diversity decreased at the high end of the carbon subsidy gradient bacterial plankton and bacteriofilm membership became increasing similar. This suggests that in enviornments that contain high amounts of labile carbon selected for fewer dominat taxa that then also came to dominate the biofilm community overtime. While we did not measure extracellular polymeric substances (EPS) direct microscopy counts showed that cells in the highest carbon treatment (C:P 500) were surrounding by what appeared to be EPS. Because biofilm EPS appeared also to increase moving from the low to high carbon treatments (Figure X) it is possible that planktonic cells were more readily incorporated into biofilms due boht to increased "stickiness" of the planktonic cells as well as the biofilm itself. While we did not observe floculating DOC which has been show to dominant high DOC environment in nature, we did measure a substanial increase in DOC in the C:P = 500 treatment which was more than 2 fold higher than any of the other treatments. In additon, comparisions of carbohydrates in the biofilm among treatments showed that carbohydrate content also generally increased with increasing carbon treatment and was significantly different in the highest vs the lowest treatment (I could show this figure or not- not sure yet)  While our study provides an additional detailed analysis of biofilm community assembly mechanisms consistent with what has been previously reported we also evaluated how resource subsidies potentially alter the seed pool (the plankton) and the biofilm community. Interestingly, biofilm community richness peaked at the intermediate treatment (C:P =100) and appeared to decrease over time although with only two time points it was unclear how pronounced this effect was. Since biomass pools for the plankton and the biofilm increased with increasing carbon subsidies the intermediate peak in OTU richness is consistent with a classic productivity-diversity relationship that has been shown for many ecosystems and communities both microbial and otherwise (REFS). Interestingly, as diversity decreased at the high end of the carbon subsidy gradient bacterial plankton and bacteriofilm membership became increasing similar. This suggests that in enviornments that contain high amounts of labile carbon selected for fewer dominat taxa that then also came to dominate the biofilm community overtime. While we did not measure extracellular polymeric substances (EPS) direct microscopy counts showed that cells in the highest carbon treatment (C:P 500) were surrounding by what appeared to be EPS. Because biofilm EPS appeared also to increase moving from the low to high carbon treatments (Figure X) it is possible that planktonic cells were more readily incorporated into biofilms due boht to increased "stickiness" of the planktonic cells as well as the biofilm itself. While we did not observe floculating DOC which has been show to dominant high DOC environment in nature, we did measure a substanial increase in DOC in the C:P = 500 treatment which was more than 2 fold higher than any of the other treatments. In additon, comparisions of carbohydrates in the biofilm among treatments showed that carbohydrate content also generally increased with increasing carbon treatment and was significantly different in the highest vs the lowest treatment (I could show this figure or not- not sure yet)  In summary this study shows mechanistic links between large scale community level dynamics the underlying populations level that drive them. Ulimately large scale changes in ecosystem processes are driven by composite effects of microbial communities actiing as a synthesis of physiological events embedded in a complex biotic and abiotic matrix.