Chuck Pepe-Ranney edited Discussion.tex  over 9 years ago

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relative differences between planktonic and biofilm diversity found in  \citet{22237539} compared to this study.  %In In  addition, for this study, it is important to note that biofilm community %richness richness  peaked at the intermediate treatment (C:P = 100) and appeared to %decrease decrease  over time %although although  with only two time points it was unclear how pronounced this effect %was was  (Figure~\ref{fig:rarefaction}). Since biomass of the plankton and the %biofilm biofilm  increased with increasing C subsidies the intermediate peak in OTU %richness richness  is consistent with a classic productivity-diversity relationship that %has has  been shown for many ecosystems and communities both microbial and %otherwise. otherwise.  However, as with other experiments with this result our experimental %design design  did not allow us to tell whether resources drove productivity that drove %changes changes  in diversity or whether resources drove diversity which altered %productivity. productivity.  Rather we note that, as diversity decreased in the highest C %treatment treatment  bacterioplankton and biofilm membership became increasingly %similar. similar.  This suggests that environments that contain high amounts of labile %C C  selected for fewer dominant taxa that came to dominate the biofilm %community, community,  overwhelming the species sorting mechanisms that appeared to %dominate dominate  biofilm community assembly in all other treatments. Similarly, while %we we  did not measure extracellular polymeric substances (EPS), direct microscopy %showed showed  that planktonic cells in the highest C treatment (C:P = 500) were %surrounded surrounded  by what appeared to be EPS. Because biofilm EPS appeared also to %increase increase  moving from the low to high C treatments %(Figure~\ref{fig:microscope}) (Figure~\ref{fig:microscope})  it is possible that more abundant planktonic %cells cells  were more readily incorporated into biofilms due both to increased %"stickiness" "stickiness"  of the planktonic cells as well as the biofilm itself. While we %did did  not observe flocculating DOC which has been shown to dominate high DOC %environments environments  in nature, we did measure a substantial increase in DOC in the C:P %= =  500 treatment which was more than 2-fold higher than any of the other %treatments. treatments.  Thus additional adhesion of the plankton and the biofilm may also %explain explain  the merging of the planktonic and biofilm bacterial membership in the %highest highest  C treatment. %\subsection{Lifestyle (biofilm or planktonic) Enriched OTUs}  %