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\subsection{Planktonic and biofilm community structure}
\subsubsection{Alpha diversity}
We evaluated alpha diversity in all treatments for both the plankton and the biofilm communities using rarefaction curves. Rarefaction curves showed bacterial and algal OTU richness was consistently higher in the biofilm compared to the planktonic communities (Figure 4). However, for both the algal community and bacterial community analysis the biofilm and planktonic communities had the fewet
OTUS OTUs in the highest C:P treatment (500) (Figure 4).
\subsubsection{Community membership biofilm versus plankton}
Not only was Membership in addition to OTU richness
was different among plankton and biofilm
treatments but membership was also distinct among the two communities. treatments. Bacterial community membership between the
bacterial plankton and biofilm communities was notably different for all treatments except for the highest carbon treatment where the plankton and biofilm communities
at day 17 were more similar to each other than any other community
and begin to resemble each other more over time (Figure
5 and Figure 6). 5). Algal plankton and biofilm communities were also composed of different OTUs however the similarity among algal plankton and biofilm communities in the highest carbon treatment was not observed as it was for the bacterial
communities(F2). communities (Figure 5).
In bacterial libraries, sequences were distributed into 636 total OTUs. 57\% of quality controlled sequences fell into the top 25 OTUs in order of decreasing sum of relative abundance across all samples. The most
differentially abundant (NOT SURE WHAT DIFF ABUND MEANS SEEMS AWKWARD) OTUs between enriched bacterial biofilm
and OTUs with respect planktonic
bacterial 16S libraries fell into the Actinobacteria, Bacteroidetes and
Proteobacteria. Proteobacteria (Figure 6). Highest absolute
log$_{2}$ fold change ratios
(IN WHAT?) of OTU means grouped by environment type (plantonic versus biofilm) were found
for OTUs enriched in planktonic
versus biofilm comparisons samples (with respect to biofilm) which is consistent
with ith the higher alpha diversity in biofilm communities compared to planktonic communiteis.
Consistent with this That is, sequence counts were spread across a greater diversty of taxa in the biofilm libraries compared to the planktonic libraries. Of the top five OTUs ordered by absolute
log$_{2}$ fold change for planktonic versus biofilm comparison environment type mean ratios one is annotated as in the \textit{Bacteroidetes}, two \textit{Gammaproteobacteria}, one \textit{Betaproteobacteria} and one \textit{Alphaproteobacteria}. Only three
bacterial OTU centroid sequences for
highly differentially abundant bacterial 16S OTUs among the top 20
OTUS ordered environment type enriched OTUs (ordered by
absolute log$_{2}$ fold change in biofilm versus planctonic
samples environment type mean ratio) share high sequence identity with cultured isolates (Table 1).
NEED TO ADD ALGAL RESULTS (WHAT'S ENRICHED)
The separation in community membership among biofilm and planktonic communitieis is supported statistically by the adonis test \cite{Anderson_2001} for both the bacterial and algal libraries(p-values 0.006 and 0.001, respectively). The environment type (biofilm or plankton) vector represents 18\% and 36\% of variance for pairwise sample distances in bacterial and algal libraries, respectively.
In contrast the only X\% of variance The adonis result is
explained by differences among resource treatments. consistent with environment type (biofilm versus plantonic) clustering along the first principal component for the algal libraries but not the bacterial libraries (Figure 5).
\subsubsection{Community membership high carbon}
Twenty-seven OTUs were differentially abundant at a false discovery rate of 10\% when comparing the high carbon treatment against all other libraries
(F7). (Figure 7). Tweleve of the 27 differentially abundant OTUs were annotated in the \textit{Gammaproteobacteria}. The next highest class-level annotation for high carbon differentially abundant OTUs was \textit{Epsilonproteobacteria} at 6. Eleven of the differentially abundant \textit{Gammaproteobacteria} OTUs were enriched in the high-carbon libraries. These 11 fell into at least 4 different orders. Only 1 OTU from the 27 was enriched in non-high carbon libraries, a gammaproteobacterium annotated as belonging in the \textit{HTCC2188} candidate order.