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We evaluated alpha diversity in all treatments for both the plankton and the biofilm communities using rarefaction curves. Rarefaction curves showed bacterial and algal OTU richness was consistently higher in the biofilm compared to the planktonic communities (Figure 4). However, for both the algal community and bacterial community analysis the biofilm and planktonic communities had the fewet OTUS in the highest C:P treatment (500) (Figure 4).  \subsubsection{Community membership biofilm versus plankton}  Not only was OTU richness different among plankton and biofilm treatments but membership was also distinct among the two communities. Bacterial community membership between the bacterial plankton and biofilm communities was notably different for all treatments except for the highest carbon treatment where the plankton and biofilm communities were more similar to each other than any other community and begin to resemble each other more over time (Figure 5 (\ref{Figure 5}  and Figure 6). Algal plankton and biofilm communities were also composed of different OTUs however the similarity among algal plankton and biofilm communities in the highest carbon treatment was not observed as it was for the bacterial communities(F2). In bacterial libraries, sequences were distributed into 636 total OTUs. 57\% of quality controlled sequences fell into the top 25 OTUs in order of decreasing sum of relative abundance across all samples. The most differentially abundant (NOT SURE WHAT DIFF ABUND MEANS SEEMS AWKWARD) OTUs between biofilm and planktonic bacterial 16S libraries fell into the Actinobacteria, Bacteroidetes and Proteobacteria. Highest absolute log$_{2}$ fold change ratios (IN WHAT?) were found in planktonic versus biofilm comparisons which is consistent with the higher alpha diversity in biofilm communities compared to planktonic communiteis. Consistent with this sequence counts were spread across a greater diversty of taxa in the biofilm libraries compared to the planktonic libraries. Of the top five OTUs ordered by absolute log$_{2}$ fold change for planktonic versus biofilm comparison one is annotated as in the \textit{Bacteroidetes}, two \textit{Gammaproteobacteria}, one \textit{Betaproteobacteria} and one \textit{Alphaproteobacteria}. Only three OTU centroid sequences for highly differentially abundant bacterial 16S OTUs among the top 20 OTUS ordered by absolute log$_{2}$ fold change in biofilm versus planctonic   samples share high sequence identity with cultured isolates (Table 1).