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Ed Hall edited Materials_and_Methods.tex
almost 9 years ago
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into the sample. Samples were then frozen and analyzed for organic C
content with a Shimadzu 500 TOC analyzer \citep{Wetzel_2000}. Biomass of all
biofilm samples were measured by difference in pre-(without biofilm) and
post-(with biofilm) weighed GF/F filters after oven drying overnight at
60C. $60\,^{\circ}{\rm C}$.
For Chl \textit{a} analysis we collected plankton on GF/F filters (Whatman,
Sigma Aldrich Cat. \# Z242489) by filtering between 500 mL and 1L from the
water column of each mesocosm for each treatment. For biofilm samples, all
biofilm was gently removed from the complete area of each coverslip (3
coverslips for each treatment per sampling event) before being placed in a test
tube for extraction with 90-95\% acetone for ~ 32 hours at
-20C $-20\,^{\circ}{\rm C}$ and analyzed
immediately after using a Turner 10-AU fluorometer \citep{Wetzel_2000}.
Bacterial abundance of the planktonic samples was analyzed using Dapi staining