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How the environment affects which microbial plankton colonize new surfaces to  form biofilms is poorly understood. Heterotrophic bacteria derive some to all  of their organic carbon (C) from photoautotrophs while simultaneously competing  for inorganic nutrients such as phosphorus (P). (P) or nitrogen (N).  Therefore, C inputs have the potential to shift the competitive balance of aquatic microbial communities by  increasing the resource space available to heterotrophic bacteria (more C)  while decreasing the resource space available to algae (less P mineral nutrients  due to increased competition from bacteria). osmotrophic heterotrophs).  To test how resource dynamics affect membership of planktonic communities and assembly of biofilm communities we amended a series  of flow-through mesocosms with C and P to achieve four target resource C:P  levels. alter the availability of C among treatments.  Each mesoscosm was fed with unfiltered seawater and incubated with sterile glass substrate for biofilm formation. We used 454 pyrosequencing of  bacterial 16S and 23S plastid genes to ask how resource driven shifts in the  pool size of each community affected community membership and structure. The