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\section{Discussion}
\subsection{Biomass Pool Size}
The goal of this study was to evaluate how changes in resource stoichiometry
affected the biomass pool size, membership and structure of planktonic and
biofilm communities. Our results suggest that C subsidies increased bacterial
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necessarily of the biofilm communities.
\subsection{Biofilm and Plankton Alpha and Beta Diversity}
Beyond changes in the biomass pool size of each community we explored how
shifts in resource C:P affected a) the membership and structure of each
community, and b) the recruitment of plankton during biofilm community
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membership in the bacterioplankton and bacterial biofilm communities the same
merging of membership was not observed for the algal biofilm and plankton
communities which had distinct membership in all treatments.
% Fakesubsubsection:We propose two potential
We propose two potential mechanisms that could result in the increased
diversity of the biofilm communities relative to the planktonic communities.
First, it is possible that the planktonic community composition of our flow
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members of the algal and bacterioplanktonic community that were in very low
abundance in the planktonic habitat but readily became major constituents of
the biofilm community.
% Fakesubsubsection:Very few studies have
Very few studies have previously evaluated the relationship among membership
and/or diversity of the plankton and the biofilm community from complex
environmental microbial communities. One notable study looked at planktonic
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sample but would explain the relative differences between planktonic and
biofilm diversity found in \citet{22237539} compared to this study.
% Fakesubsubsection:In addition, for this
In addition, for this study, it is important to note that biofilm community
richness peaked at the intermediate treatment (C:P = 100) and appeared to
decrease over time although with only two time points it was unclear how
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and biofilm bacterial membership in the highest C treatment.
\subsection{Lifestyle (biofilm or planktonic) Enriched OTUs}
There are only a few studies that attempt to compare biofilm community
composition and the overlying planktonic community \citep{Besemer_2007,
22237539, Jackson_2001, Lyautey_2005}. Those studies illustrate community
composition among the two habitats are unique with very few taxa found in both.
This is consistent with our findings in this experimental system with a natural
marine planktonic source community. In addition, our study also evaluated algal
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conditions \citep{Cho_2004, Connon_2002}.
\subsection{Conclusion}
In summary this study shows mechanistic links between large scale community
level dynamics and the underlying constituent populations that compose them. We
found that autotrophic pools and heterotrophic pools responded differently to