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Chuck Pepe-Ranney edited Results.tex
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23S plastid rRNA gene sequences were distributed into 359 total OTUs. 71\% of sequences fell into the top 25 OTUs sorted by mean relative abundance across all samples. Algal 23S plastid rRNA gene sequence libraries clustered strongly by environment type (Figure 5). Biofilm libraries were predominantly enriched in \textit{Stramenopile} OTUs whereas planktonic libraries were enriched in \textit{Haptophyceae}, \textit{Cryptophyta} and \textit{Viridiplantae} OTUs based on OTU positions in sample space (Figure 5, see Ordination Methods).
When algal OTUs are ordered by absolute environment type mean ratio (see Figure 6), 9 of the 25 OTUs are enriched in the biofilm and 16 are enriched in the planktonic samples. Eight of these 9 biofilm enriched OTUs are \textit{Stramenopiles} of class \textit{Bacillarophyta}, the remaining OTU is classified as a member of the \textit{Rhodophyta}. The 16 plantonic enriched OTUs (above) are distributed into the \textit{Viridiplantae} (5 OTUs), \textit{Cryptophyta} (4 OTUs), \textit{Haptophyceae} (4 OTUs), and \textit{Stramenopiles} (3 OTUs). Nine of the top 10 absolute environment type
algal OTU mean ratios
represented represent OTUs enriched in planktonic samples as opposed to biofilm. Environment type algal OTU mean ratios are qualitatively consistent with OTU positions in sample ordination space (see Figures 5 and 6)
The separation in community membership among biofilm and planktonic communities is supported statistically by the adonis test \cite{Anderson_2001} for both the bacterial and algal libraries (p-value 0.006 and 0.001, respectively). The environment type (biofilm or plankton) vector represents 18\% and 36\% of variance for pairwise sample distances in bacterial and algal libraries, respectively. The adonis result is consistent with environment type (biofilm versus plantonic) clustering along the first principal component for the algal libraries but not the bacterial libraries (Figure 5).