Ed Hall edited Results.tex  over 9 years ago

Commit id: 5e2c7b45b73855d4b6d23096907013efbee2b8c1

deletions | additions      

       

\subsubsection{Community membership biofilm versus plankton}  Bacterial community membership between the plankton and biofilm communities was notably different for all treatments except for the highest carbon treatment where the plankton and biofilm communities at day 17 were more similar to each other than any other community (Figure 5). Algal plankton and biofilm communities were also composed of different OTUs however the similarity among algal plankton and biofilm communities in the highest carbon treatment was not observed as it was for the bacterial communities (Figure 5).  In bacterial libraries, sequences were distributed into 636total  OTUs; 58\% of quality controlled sequences fell into the top 25 OTUs in order of decreasing sum of relative abundance across all samples. 23S plastid rRNA gene sequences were distributed into 359total  OTUs; 71\% of sequences fell into the top 25 OTUs sorted by mean relative abundance across all samples. Rank abundance curves for each mesocosm specific pair of planktonic and biofilm samples showed planktonic communities to be more sharply skewed in both the algal and bacterial datasets (Figure 9). To investigate differences in the biofilm and overlying planktonic communities we identified the OTUs that were most dramatically enriched in biofilm versus planktonic communities and vice versa. The most differentially abundanct OTUs when proportion means are calculated based on the environment type sample class (plantonic versus biofilm) were enriched in planktonic samples (with respect to biofilm) (Figure 6). This is consistent with the higher alpha diversity in biofilm communities compared to planktonic communities. That is, sequence counts were spread across a greater diversty of taxa in the biofilm libraries compared to the planktonic libraries. Of the top five environement type differentially abundant OTUs, one is annotated as in the \textit{Bacteroidetes}, two \textit{Gammaproteobacteria}, one \textit{Betaproteobacteria} and one \textit{Alphaproteobacteria}; all five are enriched in the planktonic liraries relative to biofilm. Table 1 lists the top 25 OTUs ordered by the magnitude of our differential abundance metric. Only five bacterial OTU centroid sequences for the top 25 environment type enriched OTUs share high sequence identity (>= 97\%) with cultured isolates (Table 1). The taxonomic composition of envirnoment type differentially abundant OTUs is qualitatively consistent with positions OTUs in the sample ordination space (see Figures 5 and 6).