Ed Hall edited Results.tex  almost 10 years ago

Commit id: 49c567e336c16cd4c931fca9db716090084b443f

deletions | additions      

       

When algal OTUs are ordered by absolute environment type mean ratio (see Figure 6), 9 of the 25 OTUs are enriched in the biofilm and 16 are enriched in the planktonic samples. Eight of these 9 biofilm enriched OTUs are \textit{Stramenopiles} of class \textit{Bacillarophyta}, the remaining OTU is classified as a member of the \textit{Rhodophyta}. The 16 plantonic enriched OTUs (above) are distributed into the \textit{Viridiplantae} (5 OTUs), \textit{Cryptophyta} (4 OTUs), \textit{Haptophyceae} (4 OTUs), and \textit{Stramenopiles} (3 OTUs). Nine of the top 10 absolute environment type algal OTU mean ratios represent OTUs enriched in planktonic samples as opposed to biofilm. Environment type algal OTU mean ratios are qualitatively consistent with OTU positions in sample ordination space (see Figures 5 and 6)  The separation in community membership among biofilm and planktonic communities is supported statistically by the Adonis test \cite{Anderson_2001} for both the bacterial and algal libraries (p-value 0.006 and 0.001, respectively). The environment type (biofilm or plankton) vector represents 18\% and 36\% of variance for pairwise sample distances in bacterial and algal libraries, respectively. The Adonis result is consistent with environment type (biofilm versus plantonic) clustering along the first principal component for the algal libraries but not for  the bacterial libraries (Figure 5). \subsubsection{Community membership high carbon}  Twenty-seven OTUs were enriched (using a false discovery cutoff of 10\%) when comparing the high carbon treatment (C:P = 500) against all other libraries. Twelve of the 27 high carbon enriched OTUs were annotated in the \textit{Gammaproteobacteria}. The next highest class-level annotation for high carbon differentially abundant OTUs was \textit{Epsilonproteobacteria} at 6. Eleven of the differentially abundant \textit{Gammaproteobacteria} OTUs were enriched in the high-carbon libraries. These 11 fell into at least 4 different orders. Only 1 OTU from the 27 was enriched in non-high carbon libraries, a gammaproteobacterium annotated as belonging in the \textit{HTCC2188} candidate order.