Chuck Pepe-Ranney edited Results.tex  almost 10 years ago

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\subsubsection{Community membership biofilm versus plankton}  Membership in addition to OTU richness was different among plankton and biofilm treatments. Bacterial community membership between the plankton and biofilm communities was notably different for all treatments except for the highest carbon treatment where the plankton and biofilm communities at day 17 were more similar to each other than any other community (Figure 5). Algal plankton and biofilm communities were also composed of different OTUs however the similarity among algal plankton and biofilm communities in the highest carbon treatment was not observed as it was for the bacterial communities (Figure 5).  In bacterial libraries, sequences were distributed into 636 total OTUs. 57\% 58\%  of quality controlled sequences fell into the top 25 OTUs in order of decreasing sum of relative abundance across all samples.The most enriched bacterial biofilm OTUs with respect to planktonic libraries fell into the Actinobacteria, Bacteroidetes and Proteobacteria NEED TO FIX THIS SENTENCE (Figure 6).  Highest absoluteratios of  OTUmeans of  relative abundances abundance mean ratios  grouped by environment type (plantonic versus biofilm) were found for OTUs enriched in planktonic samples (with respect to biofilm) (Figure 6)  which is consistent ith the higher alpha diversity in biofilm communities compared to planktonic communities. That is, sequence counts were spread across a greater diversty of taxa in the biofilm libraries compared to the planktonic libraries. Of the top five OTUs ordered by absolute environment type OTU mean ratios, one is annotated as in the \textit{Bacteroidetes}, two \textit{Gammaproteobacteria}, one \textit{Betaproteobacteria} and one \textit{Alphaproteobacteria}. \textit{Alphaproteobacteria}; all five are enriched in the planktonic liraries relative to biofilm.  Table 1 lists the top 20 OTUs ordered by absolute environment type mean ratios. Only three bacterial OTU centroid sequences for the top 20 environment type enriched OTUs (ordered by environment type mean ratio) share high sequence identity with cultured isolates (Table 1). Environment type OTU mean ratios are consistent with positions of OTUs in the sample ordination space (Figures (see Figures  5 and 6). 23S plastid rRNA gene sequences were distributed into 359 total OTUs. X\% 71\%  of sequences fell into the top 25 OTUs sorted by mean relative abundance across all samples. Algal 23S plastid rRNA gene sequence libraries clustered strongly by environment type (Figure 5). Biofilm libraries were predominantly enriched in \textit{Stramenopile} OTUs whereas planktonic libraries were enriched in \textit{Haptophyceae}, \textit{Cryptophyta} and \textit{Viridiplantae} OTUs(Figure 5)  based on OTU positions in sample space (see (Figure 5, see  Ordination Methods). The five most enriched algal OTUs in biofilm or plantonic libraries based on environment type OTU mean ratios belong to the X X X X (taxonomy). The separation in community membership among biofilm and planktonic communitieis is supported statistically by the adonis test \cite{Anderson_2001} for both the bacterial and algal libraries(p-values 0.006 and 0.001, respectively). The environment type (biofilm or plankton) vector represents 18\% and 36\% of variance for pairwise sample distances in bacterial and algal libraries, respectively. The adonis result is consistent with environment type (biofilm versus plantonic) clustering along the first principal component for the algal libraries but not the bacterial libraries (Figure 5).