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23S plastid rRNA gene sequences were distributed into 359 total OTUs. 71\% of sequences fell into the top 25 OTUs sorted by mean relative abundance across all samples. Algal 23S plastid rRNA gene sequence libraries clustered strongly by environment type (Figure 5). Biofilm libraries were predominantly enriched in \textit{Stramenopile} OTUs whereas planktonic libraries were enriched in \textit{Haptophyceae}, \textit{Cryptophyta} and \textit{Viridiplantae} OTUs based on OTU positions in sample space (Figure 5, see Ordination Methods). The five most environment enriched algal OTUs were similarly enriched in the planktonic samples (as opposed to biofilm).  The separation in community membership among biofilm and planktonic communities is supported statistically by the Adonis test \cite{Anderson_2001} for both the bacterial and algal libraries (p-value 0.006 and 0.001, respectively). The environment type (biofilm or plankton) vector represents 18\% and 36\% of variance for pairwise sample distances in bacterial and algal libraries, respectively. The adonis Adonis  result is consistent with environment type (biofilm versus plantonic) clustering along the first principal component for the algal libraries but not the bacterial libraries (Figure 5). \subsubsection{Community membership high carbon}  Twenty-seven OTUs were enriched (using a false discovery cutoff of 10\%) when comparing the high carbon treatment (C:P = 500) against all other libraries. Twelve of the 27 high carbon enriched OTUs were annotated in the \textit{Gammaproteobacteria}. The next highest class-level annotation for high carbon differentially abundant OTUs was \textit{Epsilonproteobacteria} at 6. Eleven of the differentially abundant \textit{Gammaproteobacteria} OTUs were enriched in the high-carbon libraries. These 11 fell into at least 4 different orders. Only 1 OTU from the 27 was enriched in non-high carbon libraries, a gammaproteobacterium annotated as belonging in the \textit{HTCC2188} candidate order.