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Chuck Pepe-Ranney edited Results.tex
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In bacterial libraries, sequences were distributed into 636 total OTUs. 58\% of quality controlled sequences fell into the top 25 OTUs in order of decreasing sum of relative abundance across all samples. Highest absolute OTU relative abundance mean ratios grouped by environment type (plantonic versus biofilm) were found for OTUs enriched in planktonic samples (with respect to biofilm) (Figure 6) which is consistent ith the higher alpha diversity in biofilm communities compared to planktonic communities. That is, sequence counts were spread across a greater diversty of taxa in the biofilm libraries compared to the planktonic libraries. Of the top five OTUs ordered by absolute environment type OTU mean ratios, one is annotated as in the \textit{Bacteroidetes}, two \textit{Gammaproteobacteria}, one \textit{Betaproteobacteria} and one \textit{Alphaproteobacteria}; all five are enriched in the planktonic liraries relative to biofilm. Table 1 lists the top 25 OTUs ordered by absolute environment type mean ratios. Only five bacterial OTU centroid sequences for the top 25 environment type enriched OTUs (ordered by absolute environment type mean ratio) share high sequence identity (>= 97\%) with cultured isolates (Table 1). Environment type OTU mean ratios are consistent with positions of OTUs in the sample ordination space (see Figures 5 and 6).
23S plastid rRNA gene sequences were distributed into 359 total OTUs. 71\% of sequences fell into the top 25 OTUs sorted by mean relative abundance across all samples. Algal 23S plastid rRNA gene sequence libraries clustered strongly by environment type (Figure 5). Biofilm libraries were predominantly enriched in \textit{Stramenopile} OTUs whereas planktonic libraries were enriched in \textit{Haptophyceae}, \textit{Cryptophyta} and \textit{Viridiplantae} OTUs based on OTU positions in sample space (Figure 5, see Ordination Methods).
The five most When algal OTUs are ordered by absolute environment
type mean ratio (see Figure 6), 9 of the 25 OTUs are enriched in the biofilm and 16 are enriched in the planktonic samples. Eight of these 9 biofilm enriched OTUs are \textit{Stramenopiles} of class \textit{Bacillarophyta}, the remaining OTU is classified as a member of the \textit{Rhodophyta}. The 16 plantonic enriched
OTUs (above) are distributed into the \textit{Viridiplantae} (5 OTUs), \textit{Cryptophyta} (4 OTUs), \textit{Haptophyceae} (4 OTUs), and \textit{Stramenopiles} (3 OTUs). Nine of the top 10 absolute environment type algal
OTU mean ratios represent OTUs
were similarly enriched in
the planktonic samples
(as as opposed to
biofilm). biofilm. Environment type algal OTU mean ratios are qualitatively consistent with OTU positions in sample ordination space (see Figures 5 and 6)
The separation in community membership among biofilm and planktonic communities is supported statistically by the Adonis test \cite{Anderson_2001} for both the bacterial and algal libraries (p-value 0.006 and 0.001, respectively). The environment type (biofilm or plankton) vector represents 18\% and 36\% of variance for pairwise sample distances in bacterial and algal libraries, respectively. The Adonis result is consistent with environment type (biofilm versus plantonic) clustering along the first principal component for the algal libraries but not the bacterial libraries (Figure 5).