deletions | additions       diff --git a/Materials_and_Methods.tex b/Materials_and_Methods.tex  index b4bdbb6..fd3e638 100644  --- a/Materials_and_Methods.tex  +++ b/Materials_and_Methods.tex 
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differential abundance analyses. We operated under the assumption that  non-cyanobacterial bacteria are predominantly heterotrophs in our mesocosm  setup and refer to the non-cyanobacterial bacterial community as  ``heterotrophs'' in the manuscript (this abstraction is useful however there are likely exceptions (i.e. -- i.e.  autotrophs among the non-cyanobacterial bacteria). All DNA sequence based results were visualized using GGPlot2  \citep{Wickham_2009}. Adonis tests and principal coordinate ordinations were performed using the Bray-Curtis similarity measure for pairwise library comparisons. Adonis tests employed the default value for number of permutations (999) ("adonis" function in Vegan R package, \citet{vegan}). Principal coordinates of OTUs were found by averaging site principal coordinate values for each OTU with OTU relative abundance values (within sites) as weights. The principal coordinate OTU weighted averages were then expanded to match the site-wise variances \citep{vegan}. \subsubsection{Identifying enriched OTUs} We used an RNA-Seq differential  expression statistical framework to find OTUs enriched in the given sample