Chuck Pepe-Ranney edited Results.tex  almost 10 years ago

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Membership between the bacterial plankton and biofilm communities was notably different for all treatments except for the highest carbon treatment where the plankton and biofilm communities were more similar to each other than any other community and begin to resemble each other more over time (F2). Algal plankton and biofilm communities were also composed of different taxa with no comparable similarities among algal communities in the highest carbon treatment as was observed for the bacterial communities(F2).  \subsubsection{Community membership}  In bacterial libraries, sequences were distributed into 636 total OTUs. 57\% of quality controlled sequences fell into the top 25 OTUs in order of decreasing sum of relative abundance across all samples.Of the top 25 OTUs, only 6 were differentially abundant by Wilcoxon Rank Sum test (p < 0.05) (cite). Of these six, four were enriched in the planktonic samples. Of the four statistically significant plankton enriched OTUs two were annotated as falling in the Oceanospirillales, one the Rhodobacterales, and the highest resolution taxonomic annotation for the last was “Betaproteobacteria”. The two aforementioned Oceanospirillales only shared 89\% sequence identity with eachother. One of the aforementioned Oceanospirillales sequences was identical to a 16S gene Marinobacterium marisflavum strain IMCC4074 and the other shared high identity (100\%) with a cultivar annotated as Gamma proteobacterium IMCC15037 from an unpublished study as well as many sequences from unpublished studies with “isolation source” annotations as “seawater” (e.g. ACCESSION). The planktonic sample enriched Rhodobacterales also shared high sequence identity (100\%) with other “seawater” derived 16S sequences from unpublished SSU rRNA gene surveys. The planktonic sample enriched Betaproteobacterium is identical to many 16S genes from uncultivated seawater microorganisms, sequences recovered in a study of bacterioplankton changes with storm disturbances (cite) as well as a cultivar annotated as “Beta proteobacterium IMCC15197” from an unpublished study (cite).