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\item   for processed data, any software used to process the data and, where possible, the source code should be made openly available.\item   for laboratory animal studies, we recommend compliance with the '\url http://www.nc3rs.org.uk/downloaddoc.asp?id=1206&page=1357&skin=0{Animal {http://www.nc3rs.org.uk/downloaddoc.asp?id=1206&page=1357&skin=0} Animal  Research: Reporting In Vivo Experiments}' Experiments'  (ARRIVE) guidelines, developed by NC3Rs to improve standards of reporting to ensure that the data from animal experiments can be fully scrutinized and utilized.\item allowances made, if any, for controlling bias or unwanted sources of variability.  Limitations of the datasets.\item