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Within the cell processes abound that can all affect the actual amount of protein produced compared to the mRNA reported by a microarray. Just a few of these may include nonsense mediated decay, inhibitory RNA, post-translational editing, protein sorting amongst cellular compartments, secondary structure in the mRNA.   Still for the largest and smallest \(\Theta\) values, the values determined might give some correlation with strong and weak translation. We will next test the leader sequences the largest Transcription Initiation Power _in vivo_ in collaboration with the glowing plant project. To this end we'll be taking the motifs for the 10 largest and some smaller \(\Theta\) motifs and installing the sequence into a plasmid that can be validated in a plant cell by quantitation of florescence from a GFP vs a control construct with its current constitutive motif sequence. When their relative strengths have been determined, the parts themselves will be placed in the golden Braid public repository. repository \cite{olo_Blanca_Granell_Orzaez_2013}.  The collection of motifs will also enable us to examine chloroplast Shine-Delgarno sequences and their relative effects on translation.