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Scott Fay,
Kyle Taylor
Synthetic biology has had profound effects on human
life, for example, providing life. It has provided more effective anti-malarial medicine, cheaper insulin,
and new useful
bio-materials. bio-materials, and greener biofuels. However, much remains to be learned
in order to synthesize
more proteins
more efficiently.
We To explore the potential of the DIY biology movement to engage in meaningful synthetic biology bioinformatics research, we developed a bioinformatics workshop to study possible determinants of protein
expression levels in plants. We extracted possible ribosome binding and translation initiation sequences and looked for correlations with experimentally determined protein levels, using publicly available data sets focusing on the widely studied plants _Oryza sativa_ and _Arabidopsis thaliana_. The working group was open to the public and met every other week for 3 hours, typically starting with a short presentation related to the session’s agenda followed by hands-on data work. We aim to develop and experimentally validate our consensus sequences for publication with hopes that our work will be useful for researchers in plant synthetic biology. We hope our experience will serve as a model for future community projects that serve the dual purpose of educating curious members of the public while also generating useful scientific results.