Bryce edited introduction.md  over 10 years ago

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Rather than approach a problem strictly as professionals, we presented an Open Source DIY workshop where scientists and the public could work together to tackle an introductory synthetic biology project resulting in a publishable outcome. The problem to be solved would need data from completely open sources, not require difficult analysis and ideally examine one or more typical bioinformatics data sources. Thus we decided to do a survey of plant translation initiation motifs which could be embodied as an open source parts list for controlling translation in metabolic engineering and synthetic biology efforts.   Plants offer many advantages as systems to do fine biological engineering [examples needed]. There is a paucity of published information, however, on how to control sets of genes working in concert. Use of small sequence motifs as ribosome binding site parts for synthetic biology has been proposed in bacteria [ \cite{Salis_Mirsky_Voigt_2009} see also: http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Anderson.] and similar parts have been produced for yeast [parts.igem.org]. So far the The  half dozen estimates of RBS parts in prokaryotic systems show that the translation of the gene can be reduced to 3% vs control, suggesting that RBS parts can be used to control gene expression in a synthetic biology effort. The Glowing Plant Project has solicited parts from the DIYBio community [reference] and effort,  so an estimate of the power of plant translation initiation motifs was conceived as a useful project.   Working meetings were posted through Counter Culture Labs and Berkeley Bio Labs (two groups with over 100 members each) on meetup.com and met every week or two over the course of three months.