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Scott Fay,
Kyle Taylor
Synthetic biology has had profound effects on human life, for example, providing more effective anti-malarial medicine, cheaper insulin, and useful bio-materials. However, much remains to be learned to synthesize more proteins efficiently. We developed a bioinformatics workshop to study possible determinants of protein levels in plants. We extracted possible ribosome binding and translation initiation sequences and looked for correlations with experimentally determined protein levels, using publicly available data sets focusing on the widely studied plants
Oryza sativa _Oryza sativa_ and
Arabidopsis thaliana. _Arabidopsis thaliana_. The working group was open to the public and met every other week for 3 hours, typically starting with a short presentation related to the session’s agenda followed by hands-on data work. We aim to develop and experimentally validate our consensus sequences for publication with hopes that our work will be useful for researchers in plant synthetic biology. We hope our experience will serve as a model for future community projects that serve the dual purpose of educating curious members of the public while also generating useful scientific results.