Kenneth Fortino edited BOD.tex  about 9 years ago

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\subsection{BOD Processing}  Removed from incubation. 15 ml removed and transferred to a 10 ml glass septum topped vial using a glass syringe. This procedure was done twice. The first vial was fixed and became T--0 and the second vial was placed on the rocker--shaker to be incubated in the dark for 5 hours. 3 ml was removed and placed into a bacterial vial with ml of gluteraldehyde. Bacterial and absorbance samples were stored in the fridge and nutrient samples